Fluorometric estimation of 4-hydroxy-3-methoxyphenylethyleneglycol sulphate in brain
نویسندگان
چکیده
منابع مشابه
The estimation of 4-hydroxy-3-methoxymandelic acid in urine.
Armstrong & McMillan (1957) identified 4hydroxy-3-methoxymandelic acid (vanilmandelic acid) in normal human urine and demonstrated that increased quantities were excreted after noradrenaline infusion and by patients with the catecholamine-secreting tumour, phaeochromocytoma. Subsequent work has established that 3methoxylation followed by oxidative deamination to 4-hydroxy-3-methoxymandelic acid...
متن کاملSimultaneous quantitation of 4-hydroxy-3-methoxymandelic (vanilmandelic) and 4-hydroxy-3-methoxyphenylacetic (homovanillic) acids in human urine.
We describe a simultaneous analysis for vanilmandelic and homovanillic acids in urine. An aliquot of a final extract, containing phenylpropriopionic acid as external standard, is dried, an internal standard (resorcinol) is added, and the mixture is silylated and chromatographed. The two compounds are identified from their characteristic retention times and from their elution behhavior in relati...
متن کاملSimultaneous liquid-chromatographic analysis for 4-hydroxy-3-methoxymandelic acid and 4-hydroxy-3-methoxyphenylacetic acid in urine.
We describe a rapid, reliable "high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5-mL aliquot of urine is adjusted to pH 6.1 and subjected to anion-exchange chromatography (Dowex AG 1-X4, 1.8 X 0...
متن کاملFluorometric estimation of antigens (antibodies).
A method for the titration of antigens based on separation by filtration through millipore filters of the complex of antigen with fluorescein isothiocyanate-labeled antibodies from free labeled anti bodies and nonspecific y-globulins is described. The fluorescence of the antigen-antibody complex is measured directly on the millipore filters using the Turner fluorometer equipped with a paper ch...
متن کاملFunctional reconstitution of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors from rat brain.
Glutamate receptors belonging to the subclass specifically activated by alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) were solubilized from rat forebrain membranes with Triton X-100 and partially purified through a series of three chromatographic steps. Specific [3H]AMPA binding increased 30-60-fold during the isolation procedure. A protein band recognized by antibodies agains...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 1972
ISSN: 0007-1188
DOI: 10.1111/j.1476-5381.1972.tb08099.x